CD49b (ITGA2) Human Monoclonal Antibody [Clone ID: F2H11]

CAT#: DDX9021A647-100

CD49b (ITGA2) human monoclonal antibody, clone F2H11

Product Images

IF staining of HPA5b+ platelets with F2H11

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  ELISA detection of HPA5b+ platelets (formerly genotyped by SSP-PCR) with F2H11

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  FACS staining of HPA5+ platelets with F2H11

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  Inhibition of collagen-induced platelet aggregation by F2H11 and B4F12

Specifications

Product Data

• Clone Name: F2H11
• Applications: FC, IF
• Recommended Dilution: Platelet phenotyping by flow cytometry, IF, ELISA, platelet aggregation studies.
  Usage recommendation:
  *This monoclonal antibody may be used between 5-25μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before use.
  
• Reactivities: Human
• Host: Human
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: CD40 activated and EBV immortalized human B lymphocytes
• Specificity: GPIa-IIa preferentially on acitvated platelets.
• Formulation: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol
  Label: Alexa Fluor 647
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Alexa Fluor 647
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: integrin subunit alpha 2
• Database Link:
  Entrez Gene 3673 Human
  UniProt ID P17301
• Background: Neonatal AlloImmune Thrombocytopenia (NAIT) is the commonest cause of severe isolated thrombocytopenia in the foetus and newborn. In Caucasians, 97% of NAIT are caused by HPA-1a or HPA- 5b alloantibodies. B4F12 was isolated after combination of CD40 activation and Epstein-Barr virus transformation of B cells isolated from a woman with high titre of anti -HPA-5b alloantibodies. B4F12 human monoclonal antibody recognizes GPIa-IIa preferentially on activated human platelets (Mérieux Y, Rigal D, Pin JJ, XVIIth Regional Congress, International Society of Blood Transfusion, Madrid 2007 ; Guillot-Chene P, Lebecque S, Rigal D, Annales Pharmaceutiques Françaises, 06/2009, 67 (3) 182-6).
• Synonyms: human platelet antigen 5b