Neurofilament (NEFL) (+ Neurofilament H) Mouse Monoclonal Antibody [Clone ID: 2F11]

CAT#: DM080X

Neurofilament (NEFL) (+ Neurofilament H) mouse monoclonal antibody, clone 2F11, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human cerebellum stained with Neurofilament antibody Cat.-No. DM080X using peroxidase conjugate and DAB chromogen. Note the cytoplasmic staining of neurons and neuronal processes.

Specifications

Product Data

• Clone Name: 2F11
• Applications: IHC, WB
• Recommended Dilution: Western Blot.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/200
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Brain.
• Reactivities: Feline, Human, Mouse, Opossum, Rabbit, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Neurofilament purified from Human brain.
• Specificity: 2F11 labels neurons, neuronal processes, and peripheral nerves as well as sympathetic ganglion cells and adrenal medulla. Cell body of neurons, containing the non-phosphorylated neurofilament, is weakly stained.
  Cellular Localization: Cytoplasmic.
• Formulation: State: Supernatant
  State: Liquid Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens neurofilament, light polypeptide (NEFL)
• Database Link:
  Entrez Gene 18039 MouseEntrez Gene 83613 RatEntrez Gene 4747 Human
• Background: Neurofilaments are the intermediate filaments of neurons and their processes. They are protein triplets composed of three major subunits of MW 68kDa, 155kDa, and 200kDa. Neurofilaments are expressed in tumors of neural origin, or displaying neuronal differentiation, such as neuroblastoma, medulloblastoma, and retinoblastoma.
• Synonyms: NF-L,NEFL, NF68, 68 kDa neurofilament protein
• Note: Mol. Wt. of Antigen: 68kDa and 200kDa (phosphorylated forms of these subunits).

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)