GM CSF (CSF2) Mouse Monoclonal Antibody [Clone ID: 429]

CAT#: DM1011BT

GM CSF (CSF2) mouse monoclonal antibody, clone 429, Biotin

Specifications

Product Data

• Clone Name: 429
• Applications: ELISA
• Recommended Dilution: ELISA: This Biotin-conjugated monoclonal antibody can be used as a Tracer/Detection antibody in Sandwich ELISA applications for Human GM-CSF detection in combination with a Capture antibody Clone 59 (Cat.-No DM1014).
  Suggested Capture Coating Dose: ~0.3 µg/ml.
  Substrate: TMB. If the above suggested conditions are followed approximately 2 pg/ml of GM-CSF in serum/ plasma or medium can be detected with an assay range of 0-500 pg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant Human GM-CSF.
• Specificity: This monoclonal antibody reacts with natural and recombinant human GM-CSF.
  Does not show any cross reaction with other human Cytokines or Growth Factors tested such as M-CSF, G-CSFR, IL-8, IL-16, IL1-beta, TGFbeta-1 and TNF-alpha.
• Formulation: 0.01M PBS, pH 7.0 ± 0.1
  Label: Biotin
  State: Liquid purified IgG fraction
  Stabilizer: 1% Gelatin
  Preservative: 0.1% Proclin-300
• Purification: Affinity Chromatography on Protein G
• Conjugation: Biotin
• Storage: Upon receipt, store (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens colony stimulating factor 2 (CSF2)
• Database Link:
  Entrez Gene 1437 Human
  UniProt ID P04141
• Background: The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.
• Synonyms: CSF2, GMCSF, Sargramostim, Molgramostin

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway