EGF Mouse Monoclonal Antibody [Clone ID: S-134]

CAT#: DM1012

EGF mouse monoclonal antibody, clone S-134, Purified

Specifications

Product Data

• Clone Name: S-134
• Applications: ELISA
• Recommended Dilution: ELISA: This antibody can be used as a Capture antibody in sandwich ELISA applications for Human EGF detection in combination with a monoclonal EGF Tracer (AM09149HR-N / AM09149BT-N, Clone S-146).
  Suggested Capture Coating Dose: 0.3 µg/well; Substrate: TMB. If the above suggested conditions are followed approximately 20 pg/mL of EGF in serum/plasma or 15 pg/mL of EGF in cell medium/urine can be detected with an assay range of 0-1000 pg/mL.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant Human EGF, 6kDa, 53 amino acid residues
• Specificity: This antibody reacts with natural and recombinant human EGF.
  Does not cross react with other Human cytokines tested such as IL-1 beta, IL-8, MCAF, TGF-beta and SAA.
• Formulation: 0.01M PBS, pH 7.2 without preservatives
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with double distillated water is recommended to adjust the final concentration to 1.00 mg/ml
• Concentration: lot specific
• Purification: Affinity Chromatograpghy on Protein G
• Conjugation: Unconjugated
• Storage: Upon receipt, store (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens epidermal growth factor (EGF), transcript variant 1
• Database Link:
  Entrez Gene 1950 Human
  UniProt ID P01133
• Background: Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein.
  Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.
• Synonyms: Urogastrone, Epidermal growth factor, URG, HOMG4

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transmembrane
• Protein Pathways: Bladder cancer, Cytokine-cytokine receptor interaction, Endocytosis, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton