IL8 (CXCL8) Mouse Monoclonal Antibody [Clone ID: I8-60]

CAT#: DM1023B

IL8 (CXCL8) mouse monoclonal antibody, clone I8-60, Biotin

Specifications

Product Data

• Clone Name: I8-60
• Applications: ELISA
• Recommended Dilution: ELISA: In combination with a monoclonal capture antibody (Cat.-No DM1022) and avidin-HRP conjugate, this Biotin conjugated antibody can be used as a tracer antibody in Sandwich ELISA applications for Human IL-8 detection.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant Human IL-8 (monocyte-derive, 72-amino acid form)
• Specificity: This antibody recognizes both, native and recombinant Human IL-8.
  No cross-reactivity with human monocyte chemotactic activating factor (MCP-1) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted).
• Formulation: 0.01M PBS, pH 7.2
  Label: Biotin
  State: Liquid purified IgG fraction
  Stabilizer: 1% Gelatin
  Preservative: 0.1% Proclin-300
• Purification: Affinitty Chromatography on Protein G
• Conjugation: Biotin
• Storage: Upon receipt, store (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens chemokine (C-X-C motif) ligand 8 (CXCL8)
• Database Link:
  Entrez Gene 3576 Human
  UniProt ID P10145
• Background: IL8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood.
  This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL8 attracts neutrophils, basophils, and T-cells, but not monocytes.
  Cystic fibrosis (CF) is characterized by severe lung inflammation. The inflammatory process is believed to be caused by massive overproduction of the proinflammatory protein IL8, and the high levels of IL8 in the CF lung are therefore believed to be the central mechanism behind CF lung pathophysiology.
• Synonyms: CXCL8, Protein 3-10C, Emoctakin, GCP1, MDNCF, MONAP, NAP1

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Bladder cancer, Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Epithelial cell signaling in Helicobacter pylori infection, NOD-like receptor signaling pathway, Pathways in cancer, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway