TGF beta 1 (TGFB1) Mouse Monoclonal Antibody [Clone ID: TB21]

CAT#: DM1047

TGF beta 1 (TGFB1) mouse monoclonal antibody, clone TB21, Azide Free

Product Images

Western blot result of the antibody used at 2ug/mL to detect platelet derived TGF-β at 12ug/lane (lane 2) 6ug/lane (lane 3), 3 ug/lane (lane 4) and 1ug/mL (lane 5) with 4- chlorol-napthol as substrate. The bottom band is the 12.5KD TGF-β monomer, the mid-band is the 25KD TGF-β dimer and the up band is TGF-β with signal peptide. Lane 1 is molecular weight markers.

Specifications

Product Data

• Clone Name: TB21
• Applications: ELISA, FN, IHC, WB
• Recommended Dilution: ELISA: This antibody recognizes both Human platelet-derived and recombinant TGF-beta in ELISA.
  Functional Assay: This antibody neutralizes TGF-beta activity in vitro, in an inhibition assay of CCL/64 cell growth and neutralizes the growth promoting action of TGF-beta in the NRK-49F colony forming assay. The effect of micro-injection of this antibody into one blastomere of two cell stage Xenopus embryos indicated that it was able to neutralize effectively the bioactivity of TGF-beta in vivo.
  Immunohistochemistry on FFPE and Frozen Sections: This antibody has been used to demonstrate TGF-beta in ovine ovarian tissue and Human breast carcinoma at a dilution of 1/1000. As a consequence of the intense staining of the erythrocytes it is possible to locate a single cell within the ovarian stroma making it useful in locating very fine capillary networks within tissue.
  Western Blotting: 5-20 ng/ml will allow visualization of 100 ng/lane of TGF-beta.
  In another Western Blot experiment the antibody used at 5-20 ng/ml, detected TGF-β at 200 ng/lane.
• Reactivities: Human, Sheep
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: TGF-beta from Human platelets.
• Specificity: This antibody detects Transforming Growth Factor beta/TGF beta.
  Western blotting demonstrated that this antibody reacts with the dimeric (25 kDa) and monomeric (12.5 kDa.) forms of TGF-β under both non-reducing and reducing conditions respectively. This antibody recognizes both human platelet-derived and recombinant TGF-β in ELISA.
• Formulation: 0.01M PBS, pH 7.0 without preservatives
  State: Azide Free
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore in 0.5 ml double distilled water.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 7040 Human
  UniProt ID P01137
• Background: TGF beta 1 (Transforming Growth Factor Beta 1) is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed as well as non neoplastic tissues, release transforming growth factors; and essentially all cells possess a specific TGF beta 1 receptor. The multimodal nature of TGF beta 1 is seen in its ability to stimulate or inhibit cellular proliferation. TGF beta 1 plays a fundamental role in tissue growth and differentiation involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function.
  The C-terminal 112 amino acids constitute the mature protein.
• Synonyms: TGFB, Transforming growth factor beta-1, TGF-beta-1