TGF beta 1 (TGFB1) Mouse Monoclonal Antibody [Clone ID: 8C4]

CAT#: DM1050

TGF beta 1 (TGFB1) mouse monoclonal antibody, clone 8C4, Azide Free

Product Images

Specifications

Product Data

• Clone Name: 8C4
• Applications: ELISA, FN, IHC
• Recommended Dilution: ELISA.
  Neutralizing Activity: This MAb neutralizes TGF beta activity in vitro in inhibition assay of CCL/64 cell growth and also neutralize the growth promoting action of TGF-beta in the NRK-49F colony forming assays. The effect of micro-injection of this MAb into one blastomere of two cell stage Xenopus embryos indicated that this MAb was able to neutralize effectively the bioactivity of TG beta in vivo.
  Immunohistochemistry: May be used in immunohistochemical techniques to locate TGF-beta within tissues.
  Western Blotting：This antibody can detect 40ng/lane of human TGF-beta on Western Blot. The blot below was stained with HRP substrate 4-Chloro-1-naphthol and H2O2.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: TGF-beta from Human platelets.
• Specificity: Western blotting demonstrated that this TGF-beta antibody reacts to either dimeric (25 kDa) or monomeric (12.5 kDa) molecules of TGF-beta under non-reducing and reducing condition respectively.
  Recognizes both Human platelet-derived and recombinant TGF beta in ELISA.
• Formulation: 0.01M PBS, pH 7.0 without preservatives.
  State: Azide Free
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with Double distillated water to adjust the final concentration to 1.00 mg/ml.
• Purification: Affinity Chromatography on Protein G.
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens transforming growth factor beta 1 (TGFB1)
• Database Link:
  Entrez Gene 7040 Human
  UniProt ID P01137
• Background: TGF beta 1 (Transforming Growth Factor Beta 1) is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed as well as non neoplastic tissues, release transforming growth factors; and essentially all cells possess a specific TGF beta 1 receptor. The multimodal nature of TGF beta 1 is seen in its ability to stimulate or inhibit cellular proliferation. TGF beta 1 plays a fundamental role in tissue growth and differentiation involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function.
  The C-terminal 112 amino acids constitute the mature protein.
• Synonyms: TGFB, Transforming growth factor beta-1, TGF-beta-1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transcription Factors
• Protein Pathways: Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Pancreatic cancer, Pathways in cancer, Renal cell carcinoma, TGF-beta signaling pathway