LOC102723996 Mouse Monoclonal Antibody [Clone ID: GM13C1]

CAT#: DM1200

LOC102723996 mouse monoclonal antibody, clone GM13C1, Purified

Product Images

Fig.2: SDS-PAGE analysis of purified GM-13C1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ug of purified GM-13C1 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.

• 

  Fig.1: FACS analysis of BOSC23 cells using GM-13C1. BOSC23 cells were transiently transfected with anexpression vector encoding either ICOSL (red curve) or anirrelevant protein (control transfectant: black curve). Binding ofGM-13C1 was detected with a PE-conjugated secondaryantibody. A positive signal was obtained only with ICOSL trans-fected cells.

Specifications

Product Data

• Clone Name: GM13C1
• Applications: ELISA, FC, WB
• Recommended Dilution: Flow Cytometry: 1.2 µg/10⁶ cells.
  Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Genetic immunisation with cDNA encoding Human ICOSL (Extracellular Domain).
• Specificity: This antibody recognizes Human CD275 / ICOS Ligand. Other species not tested.
• Formulation: Phosphate buffered saline, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction from Cell Culture Supernatant
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Database Link:
  Entrez Gene 102723996 Human
• Background: Inducible co-stimulator ligand (ICOSL) is a specific ligand on antigen-presenting cells and cells of the peripheral tissue that binds to the inducible co-stimulator receptor (ICOS)(1). ICOS belongs to the CD28/CD152 receptor family that regulates T-cell activation and function. ICOSL is expressed on monocytes, dendritic cells and B cells and can be induced by inflammatory stimuli in peripheral tissue. Binding to ICOSL delivers a co-stimulatory signal for T cell proliferation and cytokine secre-tion (2,3).
• Synonyms: B7 homolog 2, B7-like protein Gl50, B7-related protein 1, ICOSLG, B7H2, B7RP1, B7RP-1, ICOSL, KIAA0653