CEACAM5 Mouse Monoclonal Antibody [Clone ID: 26/3/13]

CAT#: DM1202

CEACAM5 mouse monoclonal antibody, clone 26/3/13, Purified

Product Images

Figure 2. FACS analysis of BOSC23 cells using 26/3/13 antibody. BOSC23 cells were transiently transfected with an expres-sion vector encoding either CEACAM5 (red curve) or an irrelevantprotein (control transfectant). Binding of 26/3/13 was detected witha PE conjugated secondary antibody. A positive signal was obtained only with CEACAM5 transfected cells.

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  Figure 1. Specificity testing of 26/3/13. BOSC23 cells were transiently transfected with expression vectorscontaining either the cDNA of CEACAM1, CEACAM5-8. Recognition of CEACAM3, 4 and of a recombinant transmembrane-anchored PSG1 fusion protein was tested on stably transfected HeLa (CEACAM3, PSG) and CHO cells (CEACAM4), respectively. Expression of the constructs was confirmed with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: TET2; PSG: Brified 26/3/13 was tested on all CEACAM transfectants. A positive signal was only obtained with CEACAM5 expressing cells (red curves).

Specifications

Product Data

• Clone Name: 26/3/13
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/200-1/400.
  Cell based ELISA with intakt, transiently transfected cells: 1/200.
  Flow Cytometry: 1.2 µg/10⁶ cells.
  Immunofluorescence: 1/200.
  Western blot: 4 µg/ml.
  Immunohistochemistry on Cryo -Sections: 1-2 µg/10⁶ cells.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Extracted protein of CEACAM5.
  Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells
• Specificity: This antibody recognizes exclusively CECAM5.
• Formulation: PBS, pH 7.2 without preservatives
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein G Chromatography
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1048 Human
• Background: CEACAM5 (CEA-related cell adhesion molecule 5, CEA) belongs to the carcinoembryonic antigen (CEA) gene family (1,2). It encodes a glycosyl phosphatidyl inositol (GPI)-linked glycoprotein with a Mr of 180,000-200,000 which is most strongly expressed on epithelial cells of the fetal and adult colon and to a minor extent on epithelial cells of the stomach and sweat glands, squamous epithelial cell of the tongue, esophagus and cervix. CEACAM5 is used as a tumor marker for early detection of recurrent disease due to its expression in adenocarcinomas of the colon, lung, breast, stomach and pancreas and in mucinous ovarian carcinomas (3). Like all members of the CEACAM family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3).
• Synonyms: CEA, Carcinoembryonic antigen