CEACAM7 Mouse Monoclonal Antibody [Clone ID: BAC2]

CAT#: DM1207

CEACAM7 mouse monoclonal antibody, clone BAC2, Purified

Product Images

Figure 1. FACS analysis of BOSC23 cells using BAC2. BOSC23 cells were transiently transfected with an expression vectorencoding either CEACAM7 (Red curve) or an irrelevant protein (control transfectant). Binding of BAC2 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM7 transfected cells.

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  Figure 2. Members of the CEACAM family were expressed on BOSC cells after transient transfection withexpression vectors containing either the cDNA of CEACAM1, 5, 6, 7, 8 or a recombinant trans-membrane-anchored PSG1 fusion protein. Recognition of CEACAM3, CEACAM4 and PSG1 was tested on stably transfected HeLa (CEACAM3, PSG) and CHO cells (CEACAM4), respectively. Expression of the con-structs was confirmed with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: TET2; PSG: Brified BAC2 was tested on all CEACAM transfectants. A positive signal was only obtained with CEACAM7 expressing cells (Red curves).

Specifications

Product Data

• Clone Name: BAC2
• Applications: ELISA, FC, IF, IHC
• Recommended Dilution: ELISA: 1/200-1/400.
  Cell based ELISA with intakt, transiently transfected cells: 1/200.
  Flow cytometry: 1.2 µg/10⁶ cells.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: CEACAM7/Fc fusion protein
• Specificity: This antibody recognizes CEACAM7.
• Formulation: Phosphate buffered saline, pH 7.2 without preservatives
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1087 Human
• Background: CEA-related cell adhesion molecule 7 (CEACAM7, CGM2) belongs to the carcinoembryonic antigen (CEA) family (1). It encodes a glycosyl phosphatidyl inositol (GPI)-linked glycoprotein which is only found on the apical surface of highly differentiated epithelial cells adult colon and on a small epithelial cell population in pancreatic ducts (2). CEACAM7 expression is completely lost upon malignant transformation already in hyperplastic polyps and early adenomas as well as in adenocarcinomas of the colon (2). Like all members of the CEACAM family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by one immunoglobulin constant-like A domain.
• Synonyms: CGM2