Granzyme K (GZMK) Mouse Monoclonal Antibody [Clone ID: GM24C3]

CAT#: DM1209

Granzyme K (GZMK) mouse monoclonal antibody, clone GM24C3, Purified

Product Images

Fig.1: FACS analysis of BOSC23 cells using GM-24C3. BOSC23 cells were transiently transfected with anexpression vector encoding either Granzyme K (red curve) oran irrelevant protein (control transfectant: black curve).Binding of GM-24C3 was detected with a PE-conjugatedsecondary antibody. A positive signal was obtained only withGranzyme K transfected cells.

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  Fig.2: Intracellular detection of granzyme B in human PBMC.FACS analysis of human PBMC using hybridoma supernatantof GM-24C3. PBMC were cultivated in the presence ofphorbolester and ionomycin subsequently fixed andpermeabilised. Binding of GM-24C3 was detected with aFITC-conjugated secondary antibody.

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  Fig3: BOSC cells were transiently transfected with expression vectors for Granzyme A, B, K, or M. Expression of the constructs was tested with an anti-myc monoclonal antibody (green curves), an irrelevant monoclonal antibody served as negative control (black curves). For specificity testing, GM-24C3 hybridoma supernatant was tested on all transfectants. A positive signal was obtained only with Granzyme K transfected cells (red curves).

Specifications

Product Data

• Clone Name: GM24C3
• Applications: ELISA, FC
• Recommended Dilution: Flow Cytometry: 1.2 µg/10⁶ cells.
  ELISA: 1/200-1/400.
  Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: GM24C3 was generated by genetic immunisation of BALB/c mice with an immunisation vector containing the Granzyme K cDNA
• Specificity: This antibody recognizes Granzyme K (GZMK).
• Formulation: PBS, pH 7.2 without preservatives
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3003 Human
• Background: Granzyme K (GrK) belongs to a family of trypsin-like serine proteases localised in the cytotoxic granules of activated T cells and NK cells. It encodes a 28 kDa serine protease whose gene is located on chromosome 5q11-12 close to the granzyme A-encoding gene. Like granzyme A, it has a trypsin-like specifity cleaving at the basic residues arginine and lysine. Granzyme K triggers rapid cell death independently of caspase activation with single-stranded DNA nicks and is primarily expressed in thymus, lung, spleen and peripheral blood leukocytes.
• Synonyms: Granzyme-3, NK-tryptase-2, Fragmentin-3, TRYP2, NK-Tryp-2
• Note: Selection: based on recognition of the complete native protein expressed on transfected mammalian cells.