IL13 receptor alpha 1 (IL13RA1) Mouse Monoclonal Antibody [Clone ID: GM1C8]

CAT#: DM1210

IL13 receptor alpha 1 (IL13RA1) mouse monoclonal antibody, clone GM1C8, Purified

Product Images

FACS analysis of BOSC23 cells using clone GM-1C8 antibody. BOSC23 cells were transiently transfected with anexpression vector encoding either IL13-R (Red curve) or anirrelevant protein (Control transfectant: black curve). Binding of GM-1C8 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with IL13-R transfected cells.

Specifications

Product Data

• Clone Name: GM1C8
• Applications: ELISA, FC, IF
• Recommended Dilution: Flow cytometry: 1.2 µg/10⁶ cells.
  The antibody is routinely tested by on BOSC cells transiently transfected with an IL-13Rα1 expression vector.
  Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
  It is expected that the antibody will be suited for all applications where recognition of the native protein is required.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: cDNA of the human IL-13Rα1 chain extracellular domain (U62858).
• Specificity: Clone GM1C8 recognises IL-13Rα1 transiently expressed on the cell surface of transfected BOSC cells as well as the native protein on monocytes (W. Luttmann, unpublished data).
  Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.01% Sodium Azide
• Concentration: lot specific
• Purification: Protein G chromatography
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3597 Human
• Background: Interleukin 13 (IL-13) is a T cell derived cytokine involved in the regulation of inflammatory and im-mune responses. IL-13Rα1 together with IL-4Rα forms a functional receptor for both IL-4 and IL-13, which is why these two cytokines share many of their biological activities. The receptor is found on human B cells, monocytes and endothelial cells. However, no functional receptor is expressed on T cells, which explains why IL-13, in contrast to IL-4, fails to induce TH2-cell differentiation.
• Synonyms: Interleukin-13 receptor alpha-1 chain, IL-13R-alpha-1, IL-13RA-1, IL13R, IL13RA, IL13 receptor alpha, CT19