IL13 receptor alpha 1 (IL13RA1) (Extracell. Dom.) Mouse Monoclonal Antibody [Clone ID: GM1E7]

CAT#: DM1227

IL13 receptor alpha 1 (IL13RA1) (Extracell. Dom.) mouse monoclonal antibody, clone GM1E7, Purified

Product Images

Figure 4: SDS-PAGE analysis of purified GM-1E7 monoclonal antibody. Lane 1: Molecular weight marker, Lane 2: 2 ug of purified GM-1E7 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.

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  Figure 2: Spectral Confocal Microscopy of CHO cells using GM-1E7 antibody. CHO cells were transiently transfected with an expression vector encoding IL13-R. Binding of GM-1E7 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (Red). Cell nuclei are stained with DAPI (Blue).

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  Figure 1: FACS analysis of BOSC23 cells using GM-1E7 antibody. BOSC23 cells were transiently transfected with an expression vector encodingeither IL13-R (Red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-1E7 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with IL13-R transfected cells.

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  Figure 3: Antagonistic Properties of GM-1E7 Dose-dependent inhibition of cellular proliferation. Samples of TF-1 cells were incubated for 72 h with 10 nM human IL-13 (closed circles) or 10 nM Human IL-4 (open circles) plus the indicated concentrations of purified GM-1E7. Antibody GM1E7 blocks IL-13-dependent cell proliferation completely, but only marginally influences IL-4-activity on TF-1 cells.

Specifications

Product Data

• Clone Name: GM1E7
• Applications: Assay, ELISA, FC, IF, WB
• Recommended Dilution: Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
  Flow cytometry: 1.2 µg/10⁶ cells.
  Immunofluorescence: 1 µg/10⁶ cells.
  Specific inhibition of IL-13-dependent proliferation of TF-1 cells by GM-1E7 (See Figure.3).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Genetic immunization with cDNA encoding Human IL13-R alpha 1 (extracellular domain).
  Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells.
• Specificity: Recognizes IL13 Receptor (IL13-R).
• Formulation: Phosphate buffered saline, pH 7.2 without preservatives
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens interleukin 13 receptor subunit alpha 1 (IL13RA1)
• Database Link:
  Entrez Gene 3597 Human
• Background: Interleukin 13 (IL-13) is a T cell derived cytokine involved in the regulation of inflammatory and im-mune responses. IL-13Ra1 together with IL-4Ra forms a functional receptor for both IL-4 and IL-13, which is why these two cytokines share many of their biological activities. The receptor is found on human B cells, monocytes and endothelial cells. However, no functional receptor is expressed on T cells, which explains why IL-13, in contrast to IL-4, fails to induce TH2-cell differentiation
• Synonyms: Interleukin-13 receptor alpha-1 chain, IL-13R-alpha-1, IL-13RA-1, IL13R, IL13RA, IL13 receptor alpha, CT19
• Note: SDS-PAGE analysis: The antibody was purified by Protein G Affinity Chromatography from cell culture supernatants and verified by SDS-Page (Figure.4).

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway