CEACAM5 (CEACAM1/5) Mouse Monoclonal Antibody [Clone ID: 4/3/17]

CAT#: DM1228

CEACAM5 (CEACAM1/5) mouse monoclonal antibody, clone 4/3/17, Purified

Product Images

Figure.1 : FACS analysis of BOSC23 cells using 4/3/17 antibody. BOSC23 cells were transiently transfected with anexpression vector encoding either CEACAM1 (Red curve) oran irrelevant protein (control transfectant). Binding of 4/3/17 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM1 transfected cells.

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  Figure.2 : FACS analysis of BOSC23 cells using 4/3/17 antibody. BOSC23 cells were transiently transfected with anexpression vector encoding either CEACAM5 (Red curve) oran irrelevant protein (control transfectant). Binding of 4/3/17 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM5 transfected cells.

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  Figure.3: Specificity testing of 4/3/17. BOSC cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, 3, 5, 6, 8 or are combinant transmembrane-anchored PSG1 fusion protein. Expression of the constructs was confirmed with monoclonal antibodies known to recognise the corresponding proteins (CEACAM1, 3, 5 and 6: D14HD11; CEACAM8: 80H3;PSG: Brified 4/3/17 was tested on all CEACAM transfectants. A positive signal was obtained with CEACAM1 and CEACAM5 expressing cells (Red curves).

Specifications

Product Data

• Clone Name: 4/3/17
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/200-1/400.
  Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
  Flow Cytometry: 1.2 µg/10⁶ cells.
  Immunohistochemistry on Cryosections: 1-2 µg/10⁶ cells.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Immunisation with extracted protein of CEACAM5.
• Specificity: Recognizes CEACAM1, 5 (BGP, CEA, CD66a/e).
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1048 Human
• Background: CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (1). It consists of seven CEACAM (CEACAM1, CEACAM3-CEACAM8) and 11 pregnancy-specific glycoprotein (PSG1-PSG11) members. The CEA family proteins belong to the immuno-globulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker (2). CEACAM1 expression is down-regulated in many tumors indicating a tumor-suppressive function. The anti-tumor effect may be due to inhibition of tumor angiogenesis, possibly by increased secretion of anti-angiogenic molecules from the cells (3). The function of CEA family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria (1).
• Synonyms: Carcinoembryonic antigen, BGP, CEA, CEACAM1, CEACAM5, CD66a,e