CD61 (ITGB3) Mouse Monoclonal Antibody [Clone ID: Y2/51]

CAT#: DM164PT

CD61 (ITGB3) mouse monoclonal antibody, clone Y2/51, Purified

Specifications

Product Data

• Clone Name: Y2/51
• Applications: FC, IHC, WB
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/100-1/200 diluted antibody to label 10e6 cells in 100 µl.
  Immunohistochemistry on Frozen and Paraffin Sections: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin prior to staining.
  Western Blotting: Detects a band/s of approximately 90kDa in platelet lysates.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: PHA stimulated peripheral blood cells.
  Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line.
• Specificity: This antibody recognises CD61.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit beta 3
• Database Link:
  Entrez Gene 3690 Human
• Background: CD61 is a 105kD glycoprotein, which is also known as integrin beta 3 chain. This molecule associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51.
  CD61 is a receptor for fibrinogen, fibronectin, vWF, vitronectin and thrombospondin.
• Synonyms: Integrin beta-3, GP3A, GPIIIa