MCP1 (CCL2) Mouse Monoclonal Antibody [Clone ID: S101]

CAT#: DM2034B

MCP1 (CCL2) mouse monoclonal antibody, clone S101, Biotin

Specifications

Product Data

• Clone Name: S101
• Applications: ELISA
• Recommended Dilution: ELISA: This Biotin-conjugated monoclonal antibody can be used as a Tracer/Detection antibody in Sandwich ELISA applications for Human MCP-1 detection in combination with a Capture antibody Clone S14 (Cat.-No DM2032) and avidin-HRP conjugate.
  Suggested Capture Coating Dose: ~0.3 µg/ml; Substrate: TMB. If the above suggested conditions are followed approximately 1.5 pg/mL of MCP-1 in serum/plasma or 4 pg/mL of MCP-1 in medium can be detected with an assay range of 0-1600 pg/mL.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant Human MCP-1
• Specificity: This monoclonal antibody reacts with natural and recombinant Human MCP-1.
  Does not react with Human interleukin-8 (IL-8) and other human cytokines tested such as interleukin-1β (IL-1β), serum amyloid A (SAA) and epidermal growth factor (EGF).
• Formulation: 0.01M PBS, pH 7.0±0.1
  Label: Biotin
  State: Liquid purified IgG fraction
  Stabilizer: 1% Gelatin
  Preservative: 0.1% Proclin-300
• Purification: Affinity Chromatography on Protein G
• Conjugation: Biotin
• Storage: Upon receipt, store (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: C-C motif chemokine ligand 2
• Database Link:
  Entrez Gene 6347 Human
  UniProt ID P13500
• Background: Monocyte chemotactic and activating factor (MCAF) is also called monocyte chemotactic protein-1 (MCP-1) and chemokine (C-C motif) ligand 2 (CCL2). It is primarily secreted by monocytes, macrophages and dendritic cells. This cytokine displays chemotactic activity for monocytes, T-cells, and basophils, but not for neutrophils or eosinophils. MCAF causes the degranulation of basophils and mast cells, and augments the activity of monocyte and macrophage. MCAF plays an important role in inflammation, angiogenesis, auto-immune diseases, renal diseases, chronic infection and granuloma formation.
• Synonyms: C-C motif chemokine 2, SCYA2, MCAF, Small-inducible cytokine A2, MCP-1, HC11, HC-11