PDPN Mouse Monoclonal Antibody [Clone ID: 18H5]

CAT#: DM3500F

PDPN mouse monoclonal antibody, clone 18H5, FITC


USD 365.00

2 Weeks*

Size
    • 50 ug

Product Images

Specifications

Product Data
Clone Name 18H5
Applications FC
Recommended Dilution FACS Analysis: Use the antibody at ≤ 0.5 µg in 100 µl volume.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Podoplanin-expressing MDCK cells.
Specificity This antibody reacts with Podoplanin (gp36). 
The unconjugated monoclonal antibody will detect native Human Podoplanin in Western Blot and on the surface of Human LEC.
Formulation PBS
Label: FITC
State: Liquid purified IgG fraction
Stabilizer: 1% BSA
Preservative: 0.02% Sodium Azide
Concentration 0.2 mg/ml
Purification Affinity Chromatography on Protein G
Conjugation FITC
Background Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential Olinked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for Ctype lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.
Synonyms Glycoprotein 36, PA2.26 antigen, T1-alpha, Aggrus, PDPN, GP36, PSEC0003, PSEC0025
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.