TIE2 (TEK) Mouse Monoclonal Antibody [Clone ID: Cl.2]

CAT#: DM3509B

TIE2 (TEK) mouse monoclonal antibody, clone Cl.2, Biotin

Product Images

Quantification of soluble and cellular TIE-2 by Sandwich ELISA: A. CM and cell lysates from HUVECs treated with PMA (25ng/ml) or left untreated were analysed by Sandwich ELISA for the concentrations of sTIE-2 or TIE-2. For Capturing anti-Human TIE-2 Cl.16 ( DM3511) was used. for the detection a mixture of biotinylated anti-human TIE-2 Cl.2 and Cl.9 ( DM3510).

Specifications

Product Data

• Clone Name: Cl.2
• Applications: ELISA, FC, WB
• Recommended Dilution: ELISA: 1-2 µg/ml.
  Western Blot.
  FACS analysis and Cell sorting: 2-5 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human soluble extracellular domain of TIE-2
• Specificity: The unconjugated Monoclonal antibody will detect native Human TIE-2/tek in ELISA experiments and on the surface of different Human cell types.
• Formulation: 0.1M Tris-Cl, 0.2M NaCl, pH 7.4
  Label: Biotin
  State: Lyophilized purified IgG fraction
  Stabilizer: 50x BSA
  Preservative: 0.02% Sodium Azide
• Reconstitution Method: Restore to a concentration of 50 µg/ml with sterile PBS solution containing 0.1% BSA.
• Purification: Protein G Chromatography
• Conjugation: Biotin
• Storage: Prior to reconstitution store at 2-8°C for one month or at -20°C for 6 months.
  Following reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 7010 Human
• Background: TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin like loops separated by three epidermal growth factor like repeats that are connected to three fibronectin type III like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell smooth muscle cell communication in venous morphogenesis.
• Synonyms: TIE2, TIE-2, Angiopoietin-1 receptor, p140 TEK