FLT4 Mouse Monoclonal Antibody [Clone ID: (3C3) 9D9]

CAT#: DM3512P

FLT4 mouse monoclonal antibody, clone (3C3) 9D9, Aff - Purified

Product Images

Western analysis of recombinant Human soluble VEGFR-3/FLT-4.

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  Immunofluorescence staining of human VEGFR-3/FLT-4 (red) in hFlt4-transfected MG63 cells. Monoclonal mouse anti-human FLT-4 clone 9D9 and polyclonal rabbit anti-human FLT-4 (D) DP3511: A and C are negative control with secondary antibody only; B (mAb) and D (pAb) with specific antibodies against human FLT-4. The experiment was performed by the research group of Dr. Wolfgang Holnthoner, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Austrian Cluster for Tissue Regeneration, Donaueschingenstrasse 13, A-1200 Vienna, Austria.

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  FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC).

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  FACS analysis with HDMVEC and HUVEC.

Specifications

Product Data

• Clone Name: (3C3) 9D9
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA: 0.5-1.5 µg/ml in direct ELISA, allows detection of 0.25-0.5 ng/well.
  FACS: 1-5 µg/ml.
  Immunofluorescence/Immunohistochemistry: 1-10 µg/ml.
  Western blot 0.5-1 µg/ml, detection limit is approximately 5 ng/lane under reducing conditions.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant Human Vascular Endothelial Growth Factor Receptor 3 (rh VEGFR-3/FLT-4) extracellular domain.
• Specificity: Detects Human VEGFR-3/FLT-4.
  Other species not tested.
• Formulation: PBS pH 7.4 without preservatives or stabilizers
  State: Aff - Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with sterile water to a concentration of 0.1-1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 2324 Human
• Background: VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk 1) and VEGFR-3 (FLT-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR-1 to -3 is almost exclusively restricted to hematopoietic precursor cells, vascular and lymphatic endothelial cells and to the monocyte/macrophage lineage. These receptors play essential roles in vasculogenesis, hematopoiesis, angiogenesis and lymphangiogenesis. The VEGFR-3 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGFR-3 is composed of a 751 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 482 aa residue cytoplasmic domain. Both VEGF-C and VEGF-D have been shown to bind and activate VEGF R3 (Flt-4). The Flt-4 gene is widely expressed in the early embryo but becomes restricted to the lymphatic endothelial a latter stage of development. It is important for lymphangiogenesis.
• Synonyms: VEGFR3, FLT4, VEGF Receptor 3