Pdpn Hamster Monoclonal Antibody [Clone ID: RTD4E10]

CAT#: DM3516P

Pdpn hamster monoclonal antibody, clone RTD4E10, Purified

Product Images

Western blot analysis using Podoplanin Antibody with recombinant Human, Mouse and Rat soluble Podoplanin. There is no cross reaction with Human Podoplanin.

Specifications

Product Data

• Clone Name: RTD4E10
• Applications: IHC, IP, WB
• Recommended Dilution: Immunohistochemistry on Frozen and Paraffin Sections: 1-10 µg/ml.
  Western blot: 1-10 µg/ml. The antibody will detect a 36-40 kDa band.
  Immunoprecipitation: 1-5 µg IgG per 1 ml lysate or reaction volume.
• Reactivities: Mouse
• Host: Hamster
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Murine thymic stromal cell line
• Specificity: The monoclonal antibody will detect Podoplanin on the surface of lymphatic endothelial cells and some epithelial cell types by immunostaining or immunohistochemistry.
  The antibody does not cross-react with Human Podoplanin.
• Formulation: PBS, pH 7.4, without preservatives or stabilizers
  State: Purified
  State: Lyophilized purified IgG fraction from Cell Culture Supernatant
• Reconstitution Method: Restore with sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Protein G Chromatography
• Database Link:
  Entrez Gene 14726 Mouse
• Background: Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a Mr 38,000 membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.
• Synonyms: Glycoprotein 36, PA2.26 antigen, T1-alpha, Aggrus, PDPN, GP36, PSEC0003, PSEC0025