VEGF Receptor 2 (KDR) Mouse Monoclonal Antibody [Clone ID: 4H3]

CAT#: DM3522B

VEGF Receptor 2 (KDR) mouse monoclonal antibody, clone 4H3, Biotin

Product Images

Figure 1. FACS analysis with primary human microvascular endothelial cells (HMVEC) using biotinylated monoclonal antibody.

Specifications

Product Data

• Clone Name: 4H3
• Applications: ELISA, FC, WB
• Recommended Dilution: ELISA: 1-10 µg/ml.
  Western Blot: 2-5 µg/ml.
  Flow Cytometry analysis and cell sorting: 2-10 µg/ml.
  Immunofluorescence/ Immunohistochemistry: 6-30 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human soluble extracellular KDR (D7) (110 kDa) protein (Cat.-No AR26018PU-N)
• Specificity: This unconjugated antibody will detect native Human VEGFR-2/KDR in ELISA experiments and on the surface of different Human cell types.
• Formulation: PBS
  Label: Biotin
  State: Lyophilized purified Ig fraction
  Stabilizer: BSA (50X)
  Preservative: 0.02% Sodium Azide
• Reconstitution Method: Restore with sterile water to a concentration of 0.1-1.0 mg/ml. Centrifuge vial prior to opening.
• Purification: Protein G Chromatography
• Conjugation: Biotin
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3791 Human
• Background: VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
• Synonyms: VEGFR2, FLK1, KDR, VEGF Receptor 2