Artn Rat Monoclonal Antibody [Clone ID: 6G32]

CAT#: DM3532P

Artn rat monoclonal antibody, clone 6G32, Purified

Specifications

Product Data

• Clone Name: 6G32
• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunohistochemistry on Frozen Sections: 1/50-1/200.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2
• Clonality: Monoclonal
• Immunogen: Purified Mouse Recombinant Artemin.
• Specificity: This antibody detects Mouse Artemin.
• Formulation: 0.2 µm filtered PBS solution
  State: Purified
  State: Lyophilized purified IgG fraction of the culture supernatant
• Reconstitution Method: Restore with 200 µl sterile PBS (final concentration = 500µg/ml).
• Purification: Affinity Chromatography on Protein A/G
• Database Link:
  Entrez Gene 11876 Mouse
• Background: Artemin is a member of the Glia Cel-lDerived Neurotrophic factor (GDNF) family ligands, which include GDNF, Persephin, Artemin, and Neurturin. GDNF family ligands are distant members of the Transforming Growth Factor β (TGFβ) superfamily. Similar to other TGFβ family proteins, Artemin is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxyterminal domain. The carboxy terminal domain of Artemin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine-knot and the single interchain disulfide bond. Biologically active Artemin is a disulfide-linked homodimer of the carboxyterminal 113 amino acid residues. Mature mouse Artemin shares 88.5% amino acid sequence similarity with human Artemin. Mature Artemin also shares approximately 40% amino acid sequence identity with the other three members of the GDNF family ligands. Bioactivities of all GDNF family ligands are mediated through a receptor complex composed of a high affinity ligand binding component (GFRα1GFRα4) and a common signaling component, cRET (receptor tyrosine kinase). Artemin prefers to bind to GFRα3 and activites the GFRα3RET. However, in the presence of RET, it can bind to GFRα1 as well. Artemin has been shown to promote the survival and growth of various peripheral and central neurons, including sympathetic and dopaminergic neurons. It may also play an important role in the development of sympathetic neurons and several organs.
• Synonyms: Enovin, Neublastin, ARTN, EVN
• Note: Predicted Molecular Weight: 24 kDa.