Nbl1 Rat Monoclonal Antibody [Clone ID: 7K12]

CAT#: DM3537P

Nbl1 rat monoclonal antibody, clone 7K12, Purified

Specifications

Product Data

• Clone Name: 7K12
• Applications: WB
• Recommended Dilution: Western Blot: 1/500 - 1/1000.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2
• Clonality: Monoclonal
• Immunogen: Mouse Recombinant DAN
• Specificity: This antibody detects Mouse DAN in Western blotting.
• Formulation: 0.2 μm filtered PBS
  State: Purified
  State: Lyophilized purified IgG fraction from Culture Supernatant
  Stabilizer: None
• Reconstitution Method: Restore with 0.2 ml sterile PBS and the final concentration is 0.5 mg/ml.
• Purification: Protein A/G Affinity Chromatography
• Database Link:
  Entrez Gene 17965 Mouse
• Background: DAN (differential screening-selected gene aberrative in neuroblastoma) was initially identified as a gene whose expression is downregulated in srctransformed rat fibroblasts. DAN has now been shown to be a prototypical member of the DAN family of secreted glycoproteins that are putative BMP antagonists. DAN family members share a cysteinerich domain that is structurally related to the cysteineknot motif found in TGFβ superfamily ligands. There are at least five mouse DAN family members, including DAN, Gremlin/DRM, Cer1 (Cerberusrelated), Dante and PRDC (protein-related to DAN and cerberus). Additional DAN family members include Xenopus Cerberus, chick Caronte, and C. elegans CeCan 1. Murine DAN binds BMP2 in immunoprecipitation experiments and acts as a BMP antagonist in Xenopus animal cap explants. The DAN family of proteins are thought to act as antagonists by binding BMP ligands and preventing their interactions with signaling receptor complexes. Recombinant mouse DAN have been shown to bind BMP4 in a functional ELISA. It is likely the various DAN family members and other TGFβ BMP antagonists including Noggin, Chordin, Follistatin, and TSG can selectively antagonize the activities of different subsets of TGFβ superfamily ligands. These antagonists represent one of the many elaborate regulatory mechanisms that have evolved to control the bioactivities of the TGFβ superfamily ligands.
• Synonyms: DAN, DAND1