TNFRSF18 Rat Monoclonal Antibody [Clone ID: 7C13]

CAT#: DM3549P

TNFRSF18 rat monoclonal antibody, clone 7C13, Purified

Product Images

4% PFA fixed/Paraffin embedded mouse Spleen, Lung and Kidney sections from LPS treated mice was subjected to immunohistochemistry staining (DAB) of GITR.

Specifications

Product Data

• Clone Name: 7C13
• Applications: FC, IHC
• Recommended Dilution: FACS Analysis: 1/50-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2
• Clonality: Monoclonal
• Immunogen: Mouse myeloma fused with spleen cells from a Rat immunized with Mouse T cells.
• Specificity: This antibody recognizes TNFRSF18/GITR.
• Formulation: PBS without preservatives or stabilizers
  State: Purified
  State: Lyophilized (0.2µm filtered) purified IgG fraction from Cell Culture Supernatant
• Reconstitution Method: Restore with 200 µl sterile PBS (final concentration 500 µg/ml).
• Purification: Protein A/G Affinity Chromatography
• Background: TNFRSF18 or GITR (glucocorticoid-induced TNF receptor family-regulated gene) is a 25 kD TNF receptor superfamily member (also known as AITR and TNFRSF18) originally identified in the mouse by comparing untreated and dexamethasone-treated murine T cell hybridoma cells. Human GITR was subsequently identified by searching an expressed sequence tag database. GITR is expressed on activated lymphocytes and is upregulated by T cell receptor engagement. The cytoplasmic domain of GITR is homologous to CD40, 4-1BB and CD27 and has been shown to interact with TRAF 1-3, but not TRAF 5 or 6. GITR signaling has been shown to regulate T cell proliferation and TCR-mediated apoptosis, and to break immunological self-tolerance. GITR appears to be highly expressed on CD4+CD25+ T regulatory cells and has been shown to induce NF-kB activation through TRAF2/NIK signaling. GITR has been proposed to be involved in the development of regulatory T cells and to regulate the activity of Th1 subsets. GITR binds GITRL, a TNF superfamily ligand expressed on endothelial cells, dendritic cells, macrophages, and B cells.
• Synonyms: AITR