Kl Rat Monoclonal Antibody [Clone ID: 4F54]

CAT#: DM3583P

Kl rat monoclonal antibody, clone 4F54, Purified

Specifications

Product Data

• Clone Name: 4F54
• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunohistochemistry on Frozen Sections: 1/50-1/200.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2
• Clonality: Monoclonal
• Immunogen: Purified Mouse Recombinant protein of Klotho
• Specificity: This antibody detects Mouse Klotho.
  Other species not tested.
• Formulation: 0.2 μm filtered solution in PBS
  State: Purified
  State: Lyophilized purified IgG fraction from Culture Supernatant
  Stabilizer: None
• Reconstitution Method: Restore with 200 µl sterile PBS and the final concentration is 500 µg/ml.
• Purification: Protein A/G Affinity Chromatography
• Database Link:
  Entrez Gene 16591 Mouse
• Background: Animal models of human aging which display the characteristic diseases associated with aging provide insight into the cause of such diseases. Recently a transgenic mouse model, called Klotho, with several premature aging phenotypes has been described. They display premature aging phenotypes such as Osteoporosis, age related skin changes, ectopic calcifications, atrophy of genital organs and thymus, emphysema and short life span. Klotho (Kl) protein associated with the Klotho mutation is a 1014 amino acid long peptide. It has a putative signal sequence at its N-terminus and a single transmembrane domain near its C-terminus, which is postulated to anchor it to the membrane. kl gene expression was observed to be tissue specific. Improvement of systemic aging phenotypes in kl/kl mice occurs even when the exogenous expression was limited to some organs, suggesting that KL associated aging is regulated through a humoral signaling pathway. KL has been reported to be localized on the cell surface when expressed on Cho cells. Human kl cDNA is expected to encode a protein of 1012 amino acids.
• Synonyms: KL, Klotho, EC 3.2.1.31