Shh (C-term) Rat Monoclonal Antibody [Clone ID: 4A37]

CAT#: DM3594P

Shh (C-term) rat monoclonal antibody, clone 4A37, Purified

Specifications

Product Data

• Clone Name: 4A37
• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2
• Clonality: Monoclonal
• Immunogen: Recombinant Mouse Sonic Hedgehog (Shh) C-Terminal fragment
• Specificity: This antibody detects Mouse Shh, it does not cross react with dhh or ihh in Western Blotting.
• Formulation: State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with 0.2 ml sterile PBS and the final concentration is 0.5 mg/ml.
• Purification: Affinity Chromatography on Protein A/G
• Database Link:
  Entrez Gene 20423 Mouse
• Background: Human Shh cDNA encodes a 462 amino acid (aa) residue (45 kDa) precursor protein with a 23 aa signal peptide. An autocatalytic cleavage reaction yields a 19 kDa (residues 24 - 197) amino-terminal fragment (Shh-N), and a 25 kDa (residues 198 - 462) carboxy-terminal domain (Shh-C). The N-terminal domain retains all known signaling capabilities, while the C-terminal domain is responsible for the intramolecular processing, acting as a cholesterol transferase that covalently transfers the cholesterol molecule to the C-terminus of Shh-N. When Shh is expressed in insect or mammalian cells, a palmitoyl group is also attached to the N-terminal cysteine of Shh-N via an amide linkage.
• Synonyms: Sonic hedgehog protein, HHG-1