Twsg1 Rat Monoclonal Antibody [Clone ID: 7D19]

CAT#: DM3604P

Twsg1 rat monoclonal antibody, clone 7D19, Purified

Specifications

Product Data

• Clone Name: 7D19
• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunohistochemistry on Frozen Sections: 1/50-1/200.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2
• Clonality: Monoclonal
• Immunogen: Purified Mouse Recombinant TSG
• Specificity: This antibody detects Mouse TSG. Other species not tested.
• Formulation: PBS
  State: Purified
  State: Lophilized purified (0.2 μm filtered) IgG fraction from Culture Supernatant
  Stabilizer: None
• Reconstitution Method: Restore with 200µl sterile PBS and the final concentration is 500µl/ml.
• Purification: Protein A/G Affinity Chromatography
• Database Link:
  Entrez Gene 65960 Mouse
• Background: Twisted gastrulation (TSG) is a secreted, cysteine-rich protein that plays a role in dorsal/ventral patterning in Drosophila and Xenopus by regulating BMP signaling. TSG was originally identified in Drosophila melanogaster and shown to be required for the differentiation of the dorsal amnioserosa cells. Studies of expression and function of Twisted Gastrulation have been performed in Drosophila and Xenopus. Xenopus TSG is expressed in the ventral regions of the embryo during gastrulation, mimicking the BMP-4 expression pattern. TSG is expressed in dorsal cells of the blastoderm embryo, where there are also high levels of activity of Dpp and Screw. Vertebrate TSGs were subsequently cloned in mouse, human, zebrafish and frog. TSG acts as an agonist for BMP signaling by modulating the inhibitory actions of the BMP antagonist, Chordin/Sog, and the cleavage properties of the metalloprotease, xolloid/tolloid. The N-terminal domain of TSG can bind BMP protein directly in vitro and shows BMP antagonist activity.
• Synonyms: Twisted gastrulation protein homolog 1