Wif1 Rat Monoclonal Antibody [Clone ID: 3M51]

CAT#: DM3608P

Wif1 rat monoclonal antibody, clone 3M51, Purified

Specifications

Product Data

• Clone Name: 3M51
• Applications: WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2
• Clonality: Monoclonal
• Immunogen: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant WIF-1.
• Specificity: This antibody detects Mouse of WIF-1 in Western blotting.
• Formulation: Lyophilized from PBS
  State: Purified
  State: Lyophilized purified IgG fractionh from Culture Supernatant
  Stabilizer: None
• Reconstitution Method: Restore with 200 µl sterile PBS and the final concentration is 500 µg/ml.
• Purification: Affinity Chromatography on Protein A/G
• Database Link:
  Entrez Gene 24117 Mouse
• Background: WIF-1 (Wnt Inhibitory Factor 1) is a secreted protein that binds to Wnt proteins and inhibits their activity. In situ hybridization analysis in Xenopus laevis and zebrafish indicate that the message is highly expressed in presomitic mesoderm, the notochord, anterior regions of the brain, branchial arches, nasal placodes, and otic vescicles. WIF-1 inhibits secondary axis induction by Wnts and promotes secondary axis induction by Chordin in Xenopus embryos. In vitro, WIF-1 binds to Drosophila Wingless and Xenopus Wnt8 proteins. WIF-1 is implicated as an early event tumor suppressor in cancers of the prostate, breast, lung, and bladder, while in other cancer types, such as colon adenocarcinoma, WIF-1 facilitates tumorigenesis. WIF-1 is also expressed in mammalian retina and may play a role in photoreceptor development. The protein is synthesized as a 379 amino acid (aa) molecule that contains a signal sequence, a 150 aa WIF domain, 5 EGF-like repeats, and a hydrophilic domain at the carboxy terminus.
• Synonyms: WIF-1, UNQ191/PRO217