LRP2 Mouse Monoclonal Antibody [Clone ID: CD7D5]

CAT#: DM3613P

LRP2 mouse monoclonal antibody, clone CD7D5, Purified

Product Images

Staining (paraffin-embedded tissue-sections fixed in formalin) of Megalin in the brush border of the proximal tubule ( )

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  Immunofluorescence staining of Megalin in the brush border of the proximal tubule ( ).

Specifications

Product Data

• Clone Name: CD7D5
• Applications: IF, IHC
• Recommended Dilution: Immunofluorescence/Immunohistochemistry: 2-5 µg/ml.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Glycoprotein 330 membrane protein fraction of isolated human glomeruli
• Specificity: This antibody recognizes Megalin/gp330.
• Formulation: PBS without preservatives or stabilizers
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml. Centrifuge vial prior to opening.
• Purification: Protein G Chromatography
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: LDL receptor related protein 2
• Database Link:
  Entrez Gene 4036 Human
• Background: Megalin, also called Lipoprotein receptor-related protein-2 (LRP2) or glycoprotein 330, is a 600-kDa transmembrane protein belonging to the LDL-receptor family. Members of this receptor family are characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF)-like repeats, YWTD repeats and an O-linked sugar domain. Megalin was first discovered in 1982 as the pathogenic antigen of Heymann nephritis. The complete cDNA sequences have been characterized for rat and human Megalin.
  The protein contains a single transmembrane domain, is known to act as an endocytic receptor and is expressed primarily on the apical membrane domain of epithelial cells. Both the protein and its mRNA have been identified in human parathyroid cells, placental cytotrophoblasts and epididymal epithelial cells. The protein is also expressed in mammary epithelia, thyroid follicular cells, yolk sacs, the ciliary body of the eye, the intestinal brush border, the male reproductive tract, uterus and oviduct, and gallbladder epithelium. In addition, immortalised foetal rat alveolar pretype II cells, adult rat type II pneumocytes and human type II cells have all been reported to express megalin. It has been proposed that in adult lung megalin may be important in supplying vitamin E to type II pneumocytes.
  Specifically, Megalin mediates the uptake of apolipoprotein J, which is a binding protein for the beta-Amyloid peptide, a peptide implicated in Alzheimer's disease. It is also involved in renal uptake of Angiotensin and the reabsorption of various molecules including calcium, vitamin B12 and vitamin D.
• Synonyms: Megalin, Glycoprotein 330, LRP-2