Ezrin (EZR) Mouse Monoclonal Antibody [Clone ID: 3C12]

CAT#: DM380

Ezrin (EZR) mouse monoclonal antibody, clone 3C12, Purified

Product Images

Western blot analysis of human lung cell extract with Ezrin antibody. DM380

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  Formalin-Fixed Paraffin-Embedded Human lung adenocarcinoma stained with Ezrin Antibody Cat.-No DM380 (Clone 3C12).

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  Formalin-Fixed Paraffin-Embedded Human lung stained with Ezrin Antibody Cat.-No DM380 (Clone 3C12).

Specifications

Product Data

• Clone Name: 3C12
• Applications: IHC, IP, WB
• Recommended Dilution: Immunoprecipitation.
  Western blot: 1/100-1/750.
  Immunohistochemistry on Paraffin Embedded Sections: dilute 1/50-1/100 in an ABC method (30 minutes at room temperature).
  Recommended Positive Control: Lung.
• Reactivities: Bovine, Hamster, Human, Kangaroo, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: BALB/C mice were immunized with a recombinant fragment of Human Ezrin corresponding to amino acid 362-585.
• Specificity: This antibody reacts with a protein of 70 kDa.
  Cellular Localization: Cell membrane.
• Formulation: State: Purified
  State: Liquid purified Ascites
  Preservative: 0.09% Sodium Azide
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 7430 Human
• Background: Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells. Ezrin is a cytoplasmic protein enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas.
• Synonyms: p81, Cytovillin, Villin-2, EZR, VIL2