CD3E Mouse Monoclonal Antibody [Clone ID: F7.2.38]

CAT#: DM422-05

CD3E mouse monoclonal antibody, clone F7.2.38, Supernatant

Product Images

Formalin fixed paraffin embedded human tonsil stained with CD3 antibody (DM422)

Specifications

Product Data

• Clone Name: F7.2.38
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use at 1/10-1/25 dilution in an ABC method (30-60 minutes at room temperature. High temperature treatment of formalin-fixed tissue sections with 1 mM EDTA buffer, pH 9.0 must be performed prior to the immunostaining.
  Immunohistochemistry on Frozen Sections (Not tested)
  Recommended Positive Control:Tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified CD3εγδ/CD3ω
• Specificity: This antibody labels CD3 and is useful tool for the identification of T cells and related neoplasms.
  Cellular Localization: Membrane and cytoplasmic.
• Formulation: State: Supernatant
  State: Liquid tissue culture supernatant
  Preservative: <0.1 Sodium Azide
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 916 Human
• Background: CD3 is first detectable in early Thymocytes and its appearance probably represents one of the earliest signs of commitment to T cell lineage. In cortical Thymocytes, the antigen is predominantly present as an intracytoplasmic constituent.
• Synonyms: T3/Leu-4