Her2 (ERBB2) Rabbit Polyclonal Antibody

CAT#: DP2012

Her2 (ERBB2) rabbit polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA.
  Western Blot.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant Human Herstatin.
  The immunization antigen (43.4 kda) is a protein containing 397 AA of recombinant Human Herstatin and one extra AA, N-terminal methionin (highlighted)
• Specificity: This Antibody reacts to Herstatin.
• Formulation: 0.05 M Phosphate buffer, 0.1 M NaCl, pH 7.2 without preservatives.
  State: Azide Free
  State: Lyophilized purified IgG fraction.
• Reconstitution Method: Add 0.05 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
• Concentration: lot specific
• Storage: Store lyophilized protein (preferably in a desiccator) at -20°C and in aliquots at -80°C.
  Reconstituted protein can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 2
• Database Link:
  Entrez Gene 2064 Human
• Background: HER-2/neu (erbB-2) encodes an 185-kDa orphan receptor tyrosine kinase that is constitutively active as a dimer and displays potent oncogenic activity when overexpressed. Herstatin, as the product of an alternative HER-2 transcript, retains intron 8. The herstatin mRNA is expressed in normal human fetal kidney and liver, but is at reduced levels relative to p185HER-2 mRNA in carcinoma cells that contain an amplified HER-2 gene. Herstatin appears to be an inhibitor of p185HER-2, because it disrupts dimers, reduces tyrosine phosphorylation of p185, and inhibits the anchorage-independent growth of transformed cells that overexpress HER-2.
• Synonyms: HER-2, NEU, p185erbB2, NGL, c-erbB-2, MNL19

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Adherens junction, Bladder cancer, Calcium signaling pathway, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer