Periostin (POSTN) Rabbit Polyclonal Antibody

CAT#: DP2019

Periostin (POSTN) rabbit polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western blot (0.1-1 µg/ml).
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin (1/100-1/5,000).
• Reactivities: Chicken, Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant Human OSF-2
  Source of Antigen: E. coli.
• Specificity: The antibody recognizes OSF-2.
• Formulation: 0.05 M Phosphate Buffer, 0.1 M NaCl, pH 7.2 without preservatives
  State: Azide Free
  State: Lyophilized (sterile filtered) purified IgG fraction
• Reconstitution Method: Add 0.05 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
• Purification: Immunoaffinity Chromatography on Immoblized Recombinant Human OSF2
• Gene Name: Homo sapiens periostin (POSTN), transcript variant 2
• Database Link:
  Entrez Gene 50706 MouseEntrez Gene 10631 Human
• Background: Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.
• Synonyms: POSTN, OSF2, OSF-2, PN
• Note:

  Antibody Content
  0.05 mg (determined by BCA method, BSA was used as a standard).

  Quality Control Test
  Indirect ELISA - to determine titer of the antibody
  SDS PAGE - to determine purity of the antibody

Reference Data

• Protein Families: Druggable Genome, Secreted Protein