LYVE1 Rabbit Polyclonal Antibody

CAT#: DP3500PS

LYVE1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western analysis of recombinant human sLYVE-1 ( DA3525) and mouse sLYVE-1 ( DA3524) using an anti-human LYVE-1 polyclonal antibody directed against the extracellular domain of human LYVE-1. There is more or less no cross reactivity with mouse LYVE-1.

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  LYVE-1 antibody staining of Human Intestine (border area of a Colon Carcinoma). The experiment has been performed by Dr. Karsten Debel, DCS, Hamburg, Germany.

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  Immunohistochemical staining of the lymphatic vessels with antihuman LYVE-1 polyclonal antibody. (A) malignant canine mammary tumor; (B) benign canine mammary tumor; (C) normal canine mammary gland tissue. The experiment was performed by the research group of Applied Veterinary Morphology - University of Antwerp.

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  Cryo sections of human colon carcinoma labeled with rabbit polyclonal antibody against human LYVE-1 (red) and human CD31 (green). A: CD31; B: LYVE-1; C: CD31/LYVE-1

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  FACS analysis with primary human dermal microvascular endothelial cells (HDMVEC).

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Specifications

Product Data

• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: Immunohistochemistry on Frozen and Paraffin Embedded Sections (1-5 µg/ml).
  Heat mediated antigen retrieval is recommended when staining paraffin embedded sections.
  Western blot (1-2 µg/ml).
  ELISA (1-15 µg/ml).
  Flow Cytometry (3-20 µg/ml).
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure recombinant Human soluble LYVE-1 produced in insect cells (Cat.-No DA3525).
  It consists of amino acid 24 (Ser) to 232 (Gly) and is fused to a C-terminal His-tag (6xHis).
• Specificity: This antibody will detect LYVE-1 on the surface of lymphatic endothelial cells by Immunohistochemistry.
  It detects a 70 kD LYVE-1 band in Western blotting.
  This antibody is not reactive with Mouse LYVE-1.
• Formulation: 5mM PBS, pH 7.2 without preservatives or stabilizers
  State: Aff - Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml
• Purification: Antigen Affinity Chromatography
• Database Link:
  Entrez Gene 10894 Human
• Background: LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosamino-glycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels.
• Synonyms: LYVE1, CRSBP-1, CRSBP1, HAR, XLKD1