PDPN Rabbit Polyclonal Antibody

CAT#: DP3512

PDPN rabbit polyclonal antibody, Azide Free

Product Images

Western blot analysis of Podoplanin expression in human lymphatic endothelial cells (LEC) and HUVECs. Total lysate of both cell types were subjected to SDS-PAGE and subsequent Western analysis with the polyclonal antibodies. The antibody recognizes a protein of about 36 kDa in total lysate from LECs but not from HUVEC.

Specifications

Product Data

• Applications: IF, IP, WB
• Recommended Dilution: Western blotting: 1-5 µg/ml.
  Immunofluorescence/Immunohistochemistry: 1-2 µg.
  Immunoprecipitation: 2-4 µg/sample.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant ectodomain of human gp36 (Podoplanin)
• Specificity: This antibody detects Human Podoplanin.
• Formulation: PBS, pH 7.4, without preservative or stabilizer
  State: Azide Free
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography on Protein A
• Database Link:
  Entrez Gene 10630 Human
• Background: Podoplanin is a highly O-glycosylated integral membrane protein that is specifically expressed in the endothelium of lymphatic capillaries but not in the blood vasculature.
  In normal skin and kidney, Podoplanin colocalized with VEGFR-3/FLT4, another marker for lymphatic endothelial cells.
• Synonyms: Glycoprotein 36, PA2.26 antigen, T1-alpha, Aggrus, PDPN, GP36, PSEC0003, PSEC0025