BMP2 (+ BMP4) Rabbit Polyclonal Antibody

CAT#: DP3515

BMP2 (+ BMP4) rabbit polyclonal antibody, Purified

Product Images

Western analysis of Human BMP-2 with a polyclonal antibody directed against Human BMP-2 derived from E. coli.

• 

  BMP-2 BioLISA using recombinant Human soluble BMP receptor 1A ( DA3521) for capturing and recombinant Human BMP-2 ( DA3502X) as standard. The polyclonal rabbit anti-human BMP-2 antibody in combination with a goat anti-rabbit-Biotin was used for detection.

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: ELISA: 2-5 µg/ml.
  Immunoprecipitation: 1-5 µg/ml.
  Western Blot: 1 µg/ml, it will detect 20 ng/lane of recombinant Human BMP-2/4-1 or BMP-2/4-2 under reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly purified Synthetic peptide C-terminal (20 amino acids) of Human BMP-2/4.
• Specificity: This polyclonal serum antibody DP3515 will detect Human BMP-2/4.
  The antibody displays cross-reaction with Human BMP-4 monomers in Western Blot.
• Formulation: PBS without preservatives
  State: Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml. Centrifuge vial prior to opening.
• Purification: Protein A Chromatography
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens bone morphogenetic protein 2 (BMP2)
• Database Link:
  Entrez Gene 650 Human
• Background: Human Bone Morphogenetic Protein-2 (BMP-2) is a disulfide-bonded homodimeric protein with an apparent molecular weight of 26 kDa. BMP-2 regulates similarly to its nearest homologue BMP-4 diverse fundamental processes during embryonic development: BMP-2 and other BMP proteins have great potential for medical therapeutic applications, in particular because they allow or at least accelerate the ossification of extensive bone lesions. The amino acid sequence of recombinant human BMP-2 starts with MQAKHKQ (position 283) containing the Met from the E. coli expression vector.
• Synonyms: Bone morphogenetic protein 2, BMP-2, BMP-2A

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transmembrane
• Protein Pathways: Acute myeloid leukemia, Basal cell carcinoma, Cytokine-cytokine receptor interaction, Endocytosis, Hedgehog signaling pathway, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer, TGF-beta signaling pathway