PROX1 (C-term) Rabbit Polyclonal Antibody

CAT#: DP3516S

PROX1 (C-term) rabbit polyclonal antibody, Purified

Product Images

Figure 1. Immunofluorescence with a Frozen Section of a Mouse embryo at day 13.5 with anti-CD31 [green] and anti-Human Prox-1 [red]. It shows a large lymphatic vessel (lv), blood vessels (arrows) and a sympathetic ganglion (sg) which is also positiv for Prox-1 (Fig.1).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blotting: 1-5 µg/ml.
  Immunohistochemistry/Immunofluorescence: 1/750-1/1,000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (> 95%) recombinant Human C-terminal part of the Homeobox Transcription Factor Prox-1 (Ala547-Glu737).
• Specificity: This antibody detect Prox-1 in the nucleus of lymphatic endothelial cells by immunostaining.
• Formulation: 5 mM PBS pH 7.2 without preservatives
  State: Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Protein A Chromatography
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 5629 Human
• Background: Prox-1 is a homeobox gene and acts as a master switch for lymphatic endothelial phenotype. Expression of Prox-1 in blood endothelial cells induces expression of other lymphatic marker genes. Together with Podoplanin, Prox-1 can be used to reliably distinguish lymphathic vessels from blood vessels. Prox-1 is expressed in CNS, eye, pancreas, liver and heart, and it is one of the most specific and reliable markers for lymphatic endothelial cells.
• Synonyms: PROX1
• Note: Always centrifuge vials before opening!