Estrogen Receptor 1 (ESR1) Rabbit Monoclonal Antibody [Clone ID: SP1]

CAT#: DRM001

Estrogen Receptor 1 (ESR1) rabbit monoclonal antibody, clone SP1, Supernatant

Product Images

DRM001

Specifications

Product Data

• Clone Name: SP1
• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry on formalin-fixed paraffin embedded sections:
  dilute 1:100 in an ABC method (30 minutes at room temperature).
  Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
  Recommended Positive Control: Breast Carcinoma
• Reactivities: Hamster, Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Synthetic peptide derived from C-terminal of human estrogen receptor
• Specificity: This antibody recognizes a protein of 67kDa, which is identified as estrogen receptor (ER).
  Cellular Localization: Nuclear.
• Formulation: State: Supernatant
  State: Liquid tissue culture supernatant containing sodium azide as preservative
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 2099 Human
• Background: ER content of breast cancer tissue is an important parameter in the prediction of prognosis and response to endocrine therapy. This antibody strongly stains the nucleus of epithelial cells in breast carcinomas. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
• Synonyms: ER alpha, Estradiol receptor, ESR1, ESR, NR3A1