Ki67 (MKI67) (C-term) Rabbit Monoclonal Antibody [Clone ID: SP6]

CAT#: DRM004

Ki67 (MKI67) (C-term) rabbit monoclonal antibody, clone SP6, Supernatant

Product Images

Formalin-fixed, paraffin-embedded human tonsil stained with Ki-67 antibody (Cat.-No DRM004) using peroxidase-conjugate and AEC. Note nuclear staining of proliferating cells.

Specifications

Product Data

• Clone Name: SP6
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Paraffin Sections: Use Ki67 antibody at a 1/50-1/100 dilution in an ABC method for 30 minutes at RT.
  Pretreatment: EDTA Buffer pH8.0
  Recommended Positive Control: Tonsil.
  Also suitable for Western blot.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide from C-terminus of Human Ki-67.
• Specificity: Reacts with Ki-67.
  Cellular Localization: Nuclear.
• Formulation: State: Supernatant
  State: Liquid Tissue Culture Supernatant
  Preservative: ≤ 0.09% Sodium Azide
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 4288 Human
• Background: Ki-67 antigen is expressed in proliferating cells but not in quiescent cells. Expression of this antigen occurs preferentially during late G1, S, G2, and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected. Consequently, Ki-67 antigen expression is used in tumor pathology to detect proliferating cells in neoplastic diseases. In cultured cells, Ki-67 is expressed in the nucleolus of interphase cells. The Ki-67 gene contains 15 exons. The Ki-67 repeat region, within which there is a 22-amino acid Ki-67 motif, is encoded by exon 13. The shorter isoform lacks exon 7. Northern blot analysis re-vealed multiple transcripts ranging from approximately 8.9 to 12.5 kb in proliferating but not quies-cent cells. Immunoblot analysis showed expression of 320- and 359-kD proteins. Sequence analy-sis predicted that the short-lived 2,896- and 3,256-amino acid protein isoforms contain potential nuclear targeting signals, over 200 potential phosphorylation sites, 19 N-myristoylation sites, 3 amidation sites, and numerous PEST sites. Antisense oligonucleotides inhibited cellular prolifera-tion in a dose-dependent manner, suggesting that Ki-67 protein expression may be an absolute requirement for cell proliferation.
• Synonyms: Ki67 antigen, MKI67, Proliferation marker