Calretinin (CALB2) Rabbit Monoclonal Antibody [Clone ID: SP13]

CAT#: DRM010

Calretinin (CALB2) rabbit monoclonal antibody, clone SP13, Supernatant

Product Images

Formalin fixed paraffin embedded human mesothelioma stained with Calretinin antibody (Cat. # DRM010).

Specifications

Product Data

• Clone Name: SP13
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Paraffin Sections: 1/100 in an ABC method (30 minutes at room temperature).
  Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
  Use mesothelioma as Positive Control.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Recombinant full-length Mouse Calretinin protein.
• Specificity: This antibody recognizes a 31.5 kD protein named Calretinin.
  Cellular Localization: Cytoplasmic.
• Formulation: State: Supernatant
  State: Liquid Tissue Culture Supernatant with 0.05% Sodium Azide as preserevative.
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: calbindin 2
• Database Link:
  Entrez Gene 794 Human
• Background: Calretinin is an intracellular calcium-binding protein belonging to the troponin C superfamily characterized by a structural mitif described as the EF-hand domain. The immunohistochemical detection of calretinin in developing cerebellum is restricted to the later stages indicated by weak staining from week 21 of gestation, in Purkinje and basket cells and in neurons of the dentate nucleus. The intensity of staining increases as the cerebellum matures. In tumors, calretinin has been detected in mesotheliomas and some pulmonary adenocarcinomas.
• Synonyms: CALB2, CAB29, 29 kDa calbindin