Histidine decarboxylase (HDC) Rabbit Polyclonal Antibody

CAT#: EUD2601

Histidine decarboxylase (HDC) rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunocytochemistry: 1/1000-1/1500 with overnight incubation at 2-8°C.
  Immunohistochemistry on Frozen and Paraffin Embedded Tissues.
  Positive Control: Stefanini-fixed frozen sections of Rat fundus.
  Western blot: The antiserum detects the 54 kDa and 73 kDa forms in addition to a 63 kDa form (Rat stomach - See Dartsch et al., 1998).
• Reactivities: Canine, Guinea Pig, Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant Histidine Decarboxylase produced in E.coli.
• Specificity: This antibody reacts with HDC.
  Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining.
• Formulation: State: Serum
  State: Undiluted Lyophilized Serum
  Preservative: 0.09% Sodium Azide
• Reconstitution Method: Dissolve the antiserum in 50-100 µl distilled water and dilute further in 0.1M PBS with 1% BSA.
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3067 Human
• Background: Histidine decarboxylase(HDC) is the enzyme that calalyses the conversion of histidine into histamine. It can be found in the histamine secreting ECL cells of some species, as well as in the mast cells.The biogenic amine histamine is an important modulator of numerous physiological processes, including neurotransmission, gastric acid secretion and smooth muscle tone.
• Synonyms: HDC