VMAT2 (SLC18A2) Guinea Pig Polyclonal Antibody
CAT#: EUD2801
VMAT2 (SLC18A2) guinea pig polyclonal antibody, Serum
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Specifications
| Product Data | |
| Applications | IF, IHC, WB |
| Recommended Dilution | Western Blot (1/500). Immunohistochemistry on Frozen Sections: 1/2000-1/3000 using FITC, with over-night incubation at 2-8°C. Positive Control: Frozen sections of Rat small intestine. |
| Reactivities | Human, Mouse, Porcine, Rat |
| Host | Guinea Pig |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide from the C-terminal of Rat VMAT2 conjugated to BSA. |
| Specificity | This antiserum recognizes monoaminergic neurons of the CNS, the ECL-cells of the stomach, as well as enteric nerve fibers. In Western blot experiments using a crude synaptic vesicle fraction of Rat brain, the 70 kDa of anti-VMaT2 is recognized (Recommended Dilution: 1/500). Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining. |
| Formulation | State: Serum State: Lyophilized Serum |
| Reconstitution Method | Dissolve in 50-100 µl distilled water, and dilute further with 0.1M PBS with 1% BSA and 0.09% Sodium Azide. |
| Storage | Store undiluted at 2-8°C for one month or (in aliquot) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Database Link | |
| Background | The vesicular monoamine transporter is responsible for the vesicular uptake of monoamines, like dopamine, norepinephrine, epinephrine, serotonin and histamine. |
| Synonyms | SVMT, VAT2 |
| Reference Data | |
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