Sodium Iodide Symporter (SLC5A5) (C-term) Rabbit Polyclonal Antibody

CAT#: EUD4101

Sodium Iodide Symporter (SLC5A5) (C-term) rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: IF, IHC
• Recommended Dilution: Immunofluorescence.
  Immunohistochemistry on Frozen Sections.
  Working Dilution: 1/1200-1/2000 using FITC conjugated secondaries.
  Incubation Time: Overnight at 2-8°C.
  Positive Control: Frozen sections of Rat thyroid.
  Suitable for studying the sensitivity of thyroid tumors to iodine treatment.
• Reactivities: Human, Porcine, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the C-terminus of Rat NIS
• Specificity: This antibody recognizes the Na⁺/I^- Symporter.
  Absorption with 50-100 µg immunogen per ml diluted antiserum abolishes staining.
• Formulation: State: Serum
  State: Lyophilized Serum
• Reconstitution Method: Dissolve with 50-100 µl distilled water, and dilute further with 0.1 M PBS with 1% BSA and 0.09% Sodium Azide
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Database Link:
  Entrez Gene 6528 Human
• Background: Human Sodium Iodide Symporter (hNIS) is responsible for iodide concentrating ability within thyroid follicular cells. It is a membrane bound glycoprotein with 13 membrane spanning domains and 14 extramembranous domains. It may represent an autoantigen in thyroid.
  Located in the cell membrane of thyroid cells, NIS can allow Sodium and Iodine flow across the membrane.
• Synonyms: Sodium-iodide cotransporter, Na+ / I- Symporter, NIS