Insulin (INS) (+Proinsulin) Guinea Pig Polyclonal Antibody

CAT#: EUD651

Insulin (INS) (+Proinsulin) guinea pig polyclonal antibody, Serum

Specifications

Product Data

• Applications: IF, IHC
• Recommended Dilution: Immunofluorescence: 1/1000-1/2000 using FITC with overnight incubation at 2-8°C.
  Immunohistochemistry on Frozen and Paraffin Sections: 1/3000-1/5000 (PAP).
  Recommended Positive Control: Formalin-fixed paraffin sections of Human pancreas.
• Reactivities: Hamster, Human, Porcine, Rat
• Host: Guinea Pig
• Clonality: Polyclonal
• Immunogen: Synthetic Human proinsulin
• Specificity: The antibody recognizes Insulin produced by the B-cells of the pancreatic islets and by insulin-producing islet cell tumors.
  Absorption with 10-100 µg proinsulin per ml diluted antiserum inactivates the antiserum, while C-peptide and Insulin only partly reduce staining.
• Formulation: State: Serum
  State: Lyophilized Undiluted Serum
• Reconstitution Method: Dissolve the antiserum in 50-100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.09% Sodium Azide
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens insulin (INS), transcript variant 1
• Database Link:
  Entrez Gene 3630 Human
• Background:
  Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.
• Synonyms: INS

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Insulin signaling pathway, Maturity onset diabetes of the young, mTOR signaling pathway, Oocyte meiosis, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Regulation of autophagy, Type I diabetes mellitus, Type II diabetes mellitus