SOX2 Goat Polyclonal Antibody

CAT#: GT15098-100

SOX2 goat polyclonal antibody

Product Images

SRY (sex determining region Y)-box 2 (SOX2) detected in immersion fixed BG01V human embryonic stem cells cultured on irradiated mouse embryonic fibroblasts for 3 hours at room temperature (Dilution: 10 ug/mL). Cells were stained using the 493-conjugated Anti-Goat IgG Secondary Antibody (green) and counterstained with DAPI (blue, inset).

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  SRY (sex determining region Y)-box 2 (SOX2) detected in immersion fixed BG01V human embryonic stem cells cultured on irradiated mouse embryonic fibroblasts for 3 hours at room temperature (Dilution: 10 ug/mL). Cells were stained using the 493-conjugated Anti-Goat IgG Secondary Antibody (green) and counterstained with DAPI (blue, inset).

Specifications

Product Data

• Applications: ELISA, IF, WB
• Recommended Dilution: Immunohistochemistry: 5-10 μg/mL.
  Western Blot: 0.1 – 0.2 μg/mL. Tested for Western blotting using recombinant protein.
  ELISA: 0.5 - 1.0 μg/mL.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Recombinant human SOX2
• Formulation: Solution in phosphate-buffered saline with 5% trehlose
  State: Aff - Purified
  State: Liquid
• Concentration: lot specific
• Gene Name: Homo sapiens SRY-box 2 (SOX2)
• Database Link:
  Entrez Gene 6657 Human
• Synonyms: Transcription factor SOX-2, ANOP3, MCOPS3, SRY-box 2
• Note: Protocol: Application Notes
  Immunocytochemistry - This antibody has been used at a concentration of 5 - 10 ug/mL to detect human SOX2. Cells were fixed with 4% paraformaldehyde in PBS at room temperature for 20 minutes, then blocked with PBS containing 0.1% Triton X-100, 1% BSA and 10% normal donkey serum at room temperature for 45 minutes. After blocking, sections were incubated with diluted primary antibody overnight at 4° C followed by Rhodamine Red coupled anti-goat IgG antibody or another appropriate secondary antibody at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA.
  Western blot - This antibody can be used at 0.1 - 0.2 ug/mL with the appropriate secondary reagents to detect human SOX2. The detection limit for recombinant human SOX2 is approximately 2 ng/lane under non-reducing and reducing conditions.

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors