Aurora B (AURKB) Mouse Monoclonal Antibody

CAT#: MO22138-100

Aurora B (AURKB) mouse monoclonal antibody

Product Images

HeLa cell cultures were stained with Aurora B kinase antibody (green). Aurora B stains midzones in anaphase and midbodies between the two daughter cells during telophase. It is therefore a useful marker of dividing cells. Counterstained is our chicken polyclonal antibody against Vimentin, (red). Blue is a DNA stain.

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  HeLa cell cultures were stained with Aurora B kinase antibody (green). Aurora B stains midzones in anaphase and midbodies between the two daughter cells during telophase. It is therefore a useful marker of dividing cells. Counterstained is our chicken polyclonal antibody against Vimentin, (red). Blue is a DNA stain.

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  Left: Western blot analysis of Aurora B in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with Aurora B. Nocodazole is a microtubule polymerization inhibitor which induces cells to halt at the G2/M phase and also induces Aurora B expression. The Aurora B monoclonal binds strongly to aurora B at 38 kDa. Right: Blot of recombinant human Aurora A, B and C were probed with [MO22138]. This antibody binds specifically to Aurora B. This is also consisted with the immunocytochemical staining of midzones and midbodies on HeLa cells.

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  Left: Western blot analysis of Aurora B in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with Aurora B. Nocodazole is a microtubule polymerization inhibitor which induces cells to halt at the G2/M phase and also induces Aurora B expression. The Aurora B monoclonal binds strongly to aurora B at 38 kDa. Right: Blot of recombinant human Aurora A, B and C were probed with [MO22138]. This antibody binds specifically to Aurora B. This is also consisted with the immunocytochemical staining of midzones and midbodies on HeLa cells.

Specifications

Product Data

• Applications: IF, IHC, WB
• Reactivities: Bovine, Chicken, Equine, Human, Mouse, Porcine, Rat
• Host: Mouse
• Isotype: IgG
• Clonality: Monoclonal
• Formulation: State: Purified
• Conjugation: Unconjugated
• Gene Name: aurora kinase B
• Database Link:
  Entrez Gene 9212 Human
  UniProt ID Q96GD4
• Synonyms: Aurora-B, Aurora/IPL1-related kinase 2, STK-1, AURKB, AIK2, AIM1, ARK2, STK12