IL7 Mouse Monoclonal Antibody

CAT#: PM1003A

IL7 mouse monoclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, FN, WB
• Recommended Dilution: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-7 (0.5 ng/ml), a concentration of 0.05-0.08 µg/ml of this antibody is required.
  ELISA: In a Sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant Human IL-7 when used with Buiotin anti-Human IL-7 antibody (PP1029B1 or PP1029B2) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
  Western Blot: To detect Hunman IL-7 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-7 is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human IL-7.
• Specificity: This antibody recognises Human IL-7.
• Formulation: PBS without preservatives.
  State: Azide Free
  State: Lyophilized (sterile filtered) purified Ig fraction.
• Reconstitution Method: Restore in sterile water to a concentration of 1.0 mg/ml.
• Purification: Affinity Chromatography on Protein A.
• Storage: Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: Homo sapiens interleukin 7 (IL7), transcript variant 1
• Database Link:
  Entrez Gene 3574 Human
• Background: Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues.
• Synonyms: IL-7

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway