RANKL (TNFSF11) Mouse Monoclonal Antibody [Clone ID: 2.1_3C12-2D11]

CAT#: PM1211P

RANKL (TNFSF11) mouse monoclonal antibody, clone 2.1_3C12-2D11, Azide Free

Product Images

Sandwich ELISA using the CD254 / RANKL Antibody Cat.-No PM1211P.

Specifications

Product Data

• Clone Name: 2.1_3C12-2D11
• Applications: ELISA
• Recommended Dilution: Sandwich ELISA: In a sandwich ELISA (assuming 100 µl/well), a concentration of 1.0-2.0 µg/ml of this antibody will detect at least 250 pg/ml of recombinant Human sRANK Ligand when used with biotinylated antigen affinity purified anti-human sRANK Ligand (Cat.-No PP1086B1 or PP1086B2) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: E.coli derived, 20.0 kDa Recombinant Human sRANK Ligand (Cat.-No PA122).
• Specificity: This antibody reacts with Human sRANKL.
  Other species not tested.
• Formulation: PBS without preservatives.
  State: Azide Free
  State: Lyophilized Sterile filtred, purified Ig fraction.
• Reconstitution Method: Restore in sterile water to a concentration of 1.0 mg/ml.
• Purification: Affinity Chromatography on Protein G
• Database Link:
  Entrez Gene 8600 Human
• Background: RANKL is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. There are three isoforms of RANKL. Human RANKL is a soluble 20 kDa polypeptide, comprising the TNF homologous region of RANKL (176 amino acid residues). This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor associated factor (TRAF) 6, which indicated that this protein may have a role in the regulation of cell apoptosis. RANKL deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.
• Synonyms: OPGL, RANK Ligand, RANKL, TRANCE, TNFSF11, ODF