Il6 Rabbit Polyclonal Antibody

CAT#: PP012P2

Il6 rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, FN, WB
• Recommended Dilution: Neutralization: b To yield one-half maximal inhibition [ND50] of the biological activity of mIL-6 (3 pg/ml), a concentration of 0.005-0.008 µg/ml of this antibody is required.
  Direct ELISA: To detect mIL-6 by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant mIL-6.
  Sandwich ELISA: To detect mIL-6 by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-0.2 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotin conjugated antibody Cat.-No PP012B, allows the detection of 0.2-0.4 ng/well of recombinant mIL-6.
  Western Blot: To detect mIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived recombinant mIL-6
• Specificity: This antibody recognises Interleukin-6 (IL-6).
• Formulation: PBS, pH 7.2 without preservatives
  State: Aff - Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography employing immobilized Murine IL-6 matrix
• Gene Name: Mus musculus interleukin 6 (Il6), transcript variant 1
• Database Link:
  Entrez Gene 16193 Mouse
• Background: Interleukin 6 (IL6) is a multifunctional 24 kDa protein originally discovered in the medium of RNA stimulated fibroblastoid cells. It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL6 has also been implicated in regulating adipose mass.
  IL6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL6 appears to interact with IL2 in the proliferation of T lymphocytes. IL6 potentiates the proliferative effect of IL3 on multipotential hematopoietic progenitors.
• Synonyms: IL-6, Interferon beta-2, IFNB2, B-cell stimulatory factor 2, BSF-2, CDF
• Note: Centrifuge vial prior to opening!