Kitl Rabbit Polyclonal Antibody

CAT#: PP018P1

Kitl rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, FN, WB
• Recommended Dilution: Neutralization:
  To yield one-half maximal inhibition [ND50] of the biological activity of mSCF (25.0 ng/ml), a concentration of 0.60-0.65 µg/ml of this antibody is required.
  ELISA:
  To detect mSCF by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant mSCF.
  Western Blot:
  To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) recombinant mSCF.
• Specificity: This antibody recognises Murine Stem Cell Factor.
• Formulation: PBS, pH 7.2 without preservatives.
  State: Aff - Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity chromatography.
• Gene Name: Mus musculus kit ligand (Kitl), transcript variant 1
• Database Link:
  Entrez Gene 17311 Mouse
• Synonyms: KITL, Kit ligand, c-Kit ligand, Stem cell factor, Mast cell growth factor, MGF
• Note: Centrifuge vial prior to opening!